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The Quantitative real-time PCR was used to determined HSA-pFSH gene copy numbers by using the CFX96 real-time system (Bio-Rad, Beijing, China) in a reaction mixture containing (in a total volume 10 l): 2 Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China), forward and reverse primers, cDNA and ddH 2 O

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Conduction through a narrow inward-rectifier K+ channel pore

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